Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

His SpinTrap^™ Talon^® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap^™ Talon^® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD ﹤ 6%. Leakage of cobalt ions from Talon^® Superflow medium is negligible.

Features and Benefits

Suitable for purification of multiple samples in parallel, such as screening. Convenient, ready-to-use single-use spin column, prepacked with 100 µL Talon^® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media. With a microcentrifuge, one run takes about 10 minutes.

General description

His SpinTrap^™ Talon^® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Legal Information

Talon is a registered trademark of Clontech

SpinTrap is a trademark of Cytiva